A fluorescent probe refers to a characteristic fluorescence in the ultraviolet-visible-near-infrared region, and its fluorescent properties (excitation and emission wavelengths, intensity, lifetime, polarization, etc.) can vary with the properties of the environment, such as polarity, refractive index, viscosity, etc. A class of fluorescent molecules that change sensitively.

A small molecular substance that changes one or several fluorescent properties by non-covalent interaction with nucleic acid (DNA or RNA), protein or other macromolecular structures. It can be used to study the properties and behavior of macromolecular substances.

Currently commonly used fluorescent probes include fluorescein probes, inorganic ion fluorescent probes, fluorescent quantum dots, and molecular beacons. In addition to the quantitative analysis of nucleic acid and protein, fluorescent probes are widely used in nucleic acid staining, DNA electrophoresis, nucleic acid molecular hybridization, quantitative PCR technology and DNA sequencing.

Fluorescence-labeled monoclonal antibody technology expands unlimited application space for flow cytometry in the study of various functional antigens and tumor gene proteins in cell membranes and cells. Fluorescent probes can be covalently attached to monoclonal antibodies via protein crosslinkers.

The most commonly used dyes for immunofluorescence labeling include fluorescein isothiocyanate (FITC), phycoerythrin (PE), and AlexaFluor series of dyes.

Application of Fluorescent Dyes in Nucleic Acid Detection

Nucleic acid fluorescent dyes stain the nucleus and quantitatively measure the fluorescence intensity emitted by the cells to determine the content of DNA and RNA in the nucleus and analyze the cell cycle and cell proliferation. There are a variety of fluorescent dyes that can stain DNA or RNA in cells. Commonly used DNA dyes include propidium iodide (PI), DAPI, Hoechst 33342, etc. RNA dyes include thiazole orange, acridine orange, etc.

About Axispharm

AxisPharm develops a broad range of cutting-edge tools and provide design/custom synthesis service for life science research including bioconjugation, diagnostics, biomaterials and therapeutic applications. As a chemistry technology company, AxisPharm has a team of highly skillful synthetic organic chemists with extensive experience in bioconjugate techniques and chemical biology research.